By S. Morris Kupchan & Aziz Karim 

Department of Chemistry, University of Virginia  

Lloydia 39(4):223-4 1976 Jul-Aug  

Aloe Vera Australia Product. Please click here… Aloe Vera Australia

Organic Aloe Vera Juice     4 x 1KG Bulk Aloe Vera Skin / Hair Gel 99% PURE   



A systematic fractionation of an ethanol-water (1:1) extract of the seeds of Rhamnus  frangula L., guided by assays for tumor-inhibitory activity, led to the isolation of Aloe  emodin (1). This compound was found to show significant antileukemic activity against the  P-388 lymphocytic leukemia in mice. A noteworthy vehicle-dependence of the testing  results is reported. In the light of this vehicle-dependence, the re-examination of other  anthraquinone derivatives is recommended.

Rhamnus frangula L. (Rhamnaceae) has been used in England and the United States to treat  cancers, and other Rhamnus species have been used similarly in folk medicine from at least  the time of Galen (circa A.D. 150) (2).

Aloe Vera Australia Product. Please click here… Aloe Vera Australia


In the course of our continuing search for tumor inhibitors of plant origin, an ethanol-water  (1:1) extract of the seeds of Rhamnus frangula L.2showed significant inhibitory activity  when tested in mice against the P-388 lymphocytic leukemia3. Fractionation of the extract,  guided by assay against the P-388 system, revealed that the inhibitory activity was  concentrated in the aqueous layer of a petroleum ether-water partition, and that the active  material was extractable by chloroform from the aqueous solution. Column chromatography  of the chloroform solubles on SilicAR CC-7 with 2.5% methanol in chloroform led to the  isolation of Aloe emodin (1) from the active chromatographic fraction. The compound was  characterized by direct comparison of its melting point, tlc, and infrared spectral  characteristics with those of an authentic sample of Aloe emodin.

Aloe emodin (1) shows significant inhibitory activity against the P-388 leukemia in mice  when administered as a suspension in acetone-Tween 80. Results corresponding to T/C  values of 133-154% were found at the optimal dose of 20 mg/kg.

In a recent review, the results of antitumor assays of 379 anthraquinone derivatives were  reported. The authors concluded that “the most noteworthy observation concerning the  anthraquinones is the relative lack of activity among the numerous derivatives tested from  this group” (4). None were found to inhibit the L-1210 leukemia in mice, and only five  showed some activity against solid tumor systems. Aloe emodin (NSC-38628) was among  the derivatives which were found to be inactive. Since the P-388 system did not number  among the tumors used in the study, our discovery of the antileukemic activity of Aloe  emodin may reflect only a unique sensitivity of this mouse leukemia toward the compound.  We note here, however, that the antileukemic activity of Aloe emodin is particularly  vehicle-dependent, and that the reproducible inhibitory activity toward the P-388 system  was manifested only when the acetone-Tween 80 suspension was used. In view of this fact,  a re-examination of other anthraquinones for potential antitumor activity, with particular  attention to possible vehicle-dependence, may be rewarded by the discovery of new and  useful structure-activity relationships.

Aloe Vera Australia Product. Please click here… Aloe Vera Australia



Extraction & Fractionation – 

Ground, dried seeds of Rhamnus frangula L. (1 kg) were extracted with ethanol-water (1:1,  7 liters) at room temperature overnight. The extract was filtered, concentrated under reduced  pressure to about 1.5 liters and freeze-dried, to yield 163 g of residue. The residue was  partitioned between petroleum ether (2 liters) and water (2 liters), whereupon 13.5 g of solid  remained undissolved and was separated by filtration. Evaporation of the petroleum ether to  dryness under reduced pressure yielded 11 g of residue. The aqueous solution was extracted  with chloroform (2 X 2 liters), and evaporation of the chloroform extract to dryness under  reduced pressure yielded 9.5 g of residue (fraction A).

Aloe Vera Australia Product. Please click here… Aloe Vera Australia


Chromatography Of Fraction A – 

A solution of fraction A (8 g) was treated with 25 g of SilicAR CC-7. The suspension was  evaporated to dryness on a rotary evaporator, and the residue was added to a column of  SilicAR CC-7 (500 g) prepared as a suspension in chloroform. The column was eluted first  with chloroform (1 liter) and then with 2.5% methanol in chloroform, and 30 X 100 ml  subfractions were collected. Subfractions were examined by tlc and those which were  similar were combined and submitted for biological testing. The aggregate of subtractions  17-25, all rich in Aloe emodin (RF 0.54), constituted the sole active fraction (B, 1.9 g).

Aloe Vera Australia Product. Please click here… Aloe Vera Australia


Isolation Of Aloe Emodin (1) – 

Active fraction B (1.5 g) was crystallized from chloroform-methanol, and recrystallization  from the same solvents yielded orange-yellow needles (700 mg), mp 223-224o; lit. mp 223- 225o(5). The melting point was not depressed by admixture of an authentic sample of Aloe emodin5. Mixture tlc and infrared spectral comparisons confirmed the identity of the two  samples.

Aloe Vera Australia Product. Please click here… Aloe Vera Australia



This work was supported by grants from the National Cancer Institute (CA-11718) and the  American Cancer Society (CI-102), and a contract with the Division of Cancer Treatment,  National Cancer Institute, National Institutes of Health, Department of Health, Education,

and Welfare (NO1-CM-12099). The excellent technical assistance of Mrs. C. Marcks is  gratefully acknowledged.

Received 8 December 1975. 



  1. Kupchan SM 1976. Novel plant-derived tumor inhibitors and their mechanisms of action. Cancer  Chemother. Rep., in press.
  2. Hartwell JL 1971. Plants used against cancer. A survey. Lloydia 34: 103.
  3. Geran RI; Greenberg NH; Macdonald MM; Schumacher AM; Abbott BJ 1972. Protocols for screening  chemical agents and natural products against animal tumors and other biological systems (third edition).  Cancer Chemother. Rep., Part 3. 3: 1. Evaluation of assay results on a statistical basis in sequential testing is  such that a material is considered active if it causes an increase in survival of treated animals (T) over controls  (C) resulting in T/C >125 percent.
  4. Driscoll JS; Hazard GF, JR.; Wood HB, JR.; Goldin A 1974. Structure-antitumor activity relationships  among quinone derivatives. Cancer Chemother. Rep., Part 2, 4(2): 1.
  5. Karrer W 1958. Konstitution und Vorkommen der organischen Pflanzcnstoffe. Birkhaüser Verlag, BaSel.  p. 517.

Aloe Vera Australia Product. Please click here… Aloe Vera Australia


1Part 113 is reference 1.

2Seeds of Rhamnus frangula L. were collected in Austria in November, 1966. We acknowledge with thanks  receipt of the dried plant material from Dr. R. E. Perdue, Jr., U.S. Department of Agriculture, in accordance  with the program developed by the National Cancer Institute. Voucher specimens are on deposit at the  Medicinal Plant Resources Laboratory, Agricultural Research Service, Beltsville, Maryland.

3 Antileukemic activity was assayed under the auspices of the National Cancer Institute, by the procedure  described in reference 3.

4 Melting points were determined with a Mettler FP2 hot-stage microscope. Infrared spectra were determined  with a Perkin-Elmer Hitachi model 257 spectrophotometer as KBr pellets. Petroleum ether refers to the  fraction of bp 60-68o. Thin-layer chromatography was carried out on silica gel 60 F-254 (E. Merck) precoated  plates, and chromatograms were visualized by spraying with an anisaldehyde-sulfuric acid spray; the  developing solvent was 5% methanol in chloroform.

5 We thank Professor H. Wagner, Universität München, for an authentic sample of Aloe emodin.

Aloe Vera Australia Product. Please click here… Aloe Vera Australia